// Automatically calculate the area of dark objects (leaves) against a white background. // 79.7619px/cm a4-200dpi. // 120.006px/cm a4-300dpi. // Change the size min to analyse smaller leaves, but risk picking up particles or noise. run("8-bit"); setAutoThreshold("Default"); //run("Threshold..."); //setThreshold(0, 146); setOption("BlackBackground", false); run("Convert to Mask"); run("Set Scale...", "distance=120.006 known=1 pixel=1 unit=cm global"); run("Analyze Particles...", "size=0.70-Infinity show=Outlines display add"); setOption(“Display Label”, true)